AAV可在心臟組織有效、持久、穩(wěn)定、安全地表達(dá)外源基因,因而在心臟基因治療策略中占據(jù)獨(dú)特優(yōu)勢(shì)。在使用rAAV進(jìn)行心臟研究時(shí),正確選擇AAV血清型、啟動(dòng)子以及注射方式是實(shí)驗(yàn)順利開(kāi)展的必備條件。今天小V就和大家一起來(lái)學(xué)習(xí)一下AAV在心臟研究中的優(yōu)化策略,以提高AAV的基因轉(zhuǎn)移效率。
+AAV在心臟研究中的靶向策略【應(yīng)用篇】
+AAV在心臟研究中的應(yīng)用及注射方法
+維真AAV病毒在高血壓腦出血中的應(yīng)用
心臟方面:
1.
Hypertension. (IF=7.713). Liu, Wen, Li, et.al. (2020). Oleic Acid Attenuates Ang II(Angiotensin II)-Induced Cardiac Remodeling by Inhibiting FGF23(Fibroblast Growth Factor 23.[中國(guó)醫(yī)學(xué)科學(xué)院阜外醫(yī)院 AAV9-Fgf23&AAV9-GFP 高血壓心臟重構(gòu)]
2.
International Journal of Molecular Medicine. (IF=3.098). Huang, et.al. (2019). Redd1 protects against post-infarction cardiac dysfunction by targeting apoptosis and autophagy.[華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬協(xié)和醫(yī)院 AAV9-Redd1 & AAV9-GFP 心機(jī)梗死MI]
3.
Frontiers in Pharmacology. (IF=4.225). Shen, et.al. (2019). Downregulation of miR-146a Contributes to Cardiac Dysfunction Induced by the Tyrosine Kinase Inhibitor Sunitinib.[河北醫(yī)科大學(xué) AAV9-cTNT-GFP-miR-146a & AV-miR-146a SNT-誘導(dǎo)的心臟收縮障礙]
4.
Journal of Molecular and Cellular Cardiology. (IF=4.133). Wu, et.al. (2019). The protective effect of high mobility group protein HMGA2 in pressure overload-induced cardiac remodeling.[武漢大學(xué) AAVs & Ads心臟重構(gòu)]
5.
Biochemical and Biophysical Research Communications. (IF=2.985). Yang, et.al. (2018). Vaspin alleviates myocardial ischaemia/reperfusion injury via activating autophagic ?ux and restoring lysosomal function.[山東大學(xué)齊魯醫(yī)院 AAV-vaspin & Ad-RFP-GFP-LC3 心臟]
血管方面:
Arteriosclerosis, Thrombosis, and Vascular Biology. (IF=6.604). Zhao, et.al. (2019). ALDH2 (Aldehyde Dehydrogenase 2) Protects Against Hypoxia-Induced Pulmonary Hypertension.[山東大學(xué)齊魯醫(yī)院 AAV2-SM22α-ALDH2 & AAV1-ICAM2-ALDH2 低氧性肺動(dòng)脈高壓HPH]
Journal of Molecular and Cellular Cardiology. (IF=4.133). Sun, et.al. (2019). LncRNA H19 promotes vascular inflammation and abdominal aortic aneurysm formation by functioning as a competing endogenous RNA.[南方醫(yī)科大學(xué)南方醫(yī)院 AAV9-H19 腹主動(dòng)脈瘤AAA]
Journal of Cerebral Blood Flow & Metabolism. (IF=5.681). Li, et.al. (2019). Overexpression of LH3 reduces the incidence of hypertensive intracerebral hemorrhage in mice.[中國(guó)醫(yī)學(xué)科學(xué)院阜外醫(yī)院 AAV9-LH3-p2A-GFP 高血壓性腦出血ICH]
Arteriosclerosis, Thrombosis, and Vascular Biology. (IF=6.604). Zhong, et.al. (2019). SM22α (Smooth Muscle 22α) Prevents Aortic Aneurysm Formation by Inhibiting Smooth Muscle Cell Phenotypic Switching Through Suppressing Reactive Oxygen Species/NF-κB (Nuclear Factor-κB).[南方醫(yī)科大學(xué)南方醫(yī)院 AAV9-SM22α 腹主動(dòng)脈瘤AAA]
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